HIGHLIGHTS OF SGRNA

Low cytotoxicity: Low impurity (residual substance) content;

High editing efficiency: Increased experimental success rate;

Stable quality: Strict quality control between batches;

No DNA interference: No unnecessary gene fragment insertion.

SERVICE DETAILS OF SGRNA

Service Modifications length(nt) Quantity Turnaround Time* Purification QC Deliverable

Cas9 sgRNA-OPC First and last 3 bases with 2'-OMe and PS 97~103 1.5 nmol 5 OPC UV& MS Tube or customized lyophilized RNA

COA report (electronic)

3 nmol

5 nmol

Cas9 sgRNA-HPLC 1.5 nmol 10~12

3 nmol

5 nmol

Cas9 crRNA ~36 5 nmol 5~7 HPLC UV& MS& HPLC

Cas9 tracrRNA ~67 3 nmol

LbaCasl2a crRNA Bases 1-3 and 29-40 (5'-end) and base 32 (3'-end) with 2'-OMe, first and last 3 bases with PS ~40 5 nmol

AsCasl2a crRNA ~40 5 nmol

LwCas13a crRNA None ~55 5 nmol

PspCasl3b crRNA ~56 5 nmol

RxCasl3d crRNA ~43 5 nmol

CASES OF SGRNA

The sample of this custom RNA oligos is a 100 nt sgRNA with three 2'-OMe modifications at both ends. Mass spectrometry and chromatography analyses confirm its sequence accuracy and a high purity of 96.54%.

For more information about sgrna plasmid, please feel free to contact us!

AAV, LV, and AdV virus packaging, library construction, and cell line development, providing ready-to-use, reliable tools to accelerate gene research and functional studies.

VIRUS PACKAGING

OVERVIEW

Virus packaging services offer high-quality, ready-to-use viral vectors for gene delivery, functional genomics, gene therapy studies, and CRISPR or shRNA library experiments. Our services cover a comprehensive suite of solutions for genetic research, including Adeno-Associated Virus (AAV) packaging, Lentivirus (LV) packaging, Adenovirus (AdV) packaging, library construction, and cell line construction. Our viral vectors are produced under strict quality control, ensuring high titer, purity, and safety, while offering fast production, reproducibility, scalability, and endotoxin-free preparations. Combined with expert support for custom library generation and reliable cell line construction, we provide end-to-end solutions that streamline your experiments, accelerate research workflows, and allow you to focus on scientific discovery.

POPULAR SERVICES OF VIRUS PACKAGING

AAV Packaging

High-quality adeno-associated virus vectors for efficient and safe gene delivery.

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LV Packaging

Lentiviral vectors with high titer and reproducibility for stable gene expression.

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AdV Packaging

Adenoviral vectors ideal for transient gene expression and large transgene delivery.

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Library Construction

Customized CRISPR or shRNA libraries for functional genomics and screening studies.

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Cell Line Construction

Stable or inducible cell lines tailored for gene overexpression, knockout, or knock-in experiments.

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KEY ADVANTAGES OF VIRUS PACKAGING

High Quality and Safety

Viral vectors are produced under strict quality control with high titer, purity, and endotoxin-free preparations.

Fast Turnaround

Gene synthesis and viral vector packaging delivered in as fast as 15 days.

Comprehensive Solutions

Coverage of AAV, LV, and AdV packaging, plus customized library construction and stable cell line generation for diverse applications.

Research Acceleration

Ready-to-use vectors and expert technical support streamline experimental workflows, enabling faster functional genomics and gene therapy studies.

SERVService Description Titer / Quantity Turnaround Time Applications

AAV Packaging Adeno-Associated Virus packaging service for stable and long-term gene delivery. High-titer, purity-controlled, endotoxin-free viral prep 12~18  business days Gene therapy studies, functional genomics, in vivo experiments

LV Packaging Lentivirus packaging service for efficient gene delivery in dividing and non-dividing cells. High-titer, purity-controlled, endotoxin-free viral prep 15~25 calendar days CRISPR screening, stable cell line generation

AdV Packaging Adenovirus packaging service for high expression and transient gene delivery. High-titer, purity-controlled, endotoxin-free viral prep 55 business days Overexpression studies, vaccine research

Library Construction Custom sgRNA libraries and site-saturation mutagenesis libraries tailored to functional genomics and screening studies. Virus ≥1 mL, >1E+8 TU/mL (typical) Inquiry Genome-wide or pathway-specific functional screens

Cell Line Construction Generation of stable cell lines using packaged viral vectors. Custom deliverables depending on design Inquiry Long-term functional studies, disease modelingICE DETAILS OF VIRUS PACKAGING

WORKFLOW OF VIRUS PACKAGING

Sequence Design & Synthesis: Design and synthesize the DNA sequence (gene or sgRNA cassette) of interest.

Plasmid Preparation: Clone the gene or sgRNA cassette into an appropriate transfer plasmid and prepare high-quality DNA.

Virus Packaging: Introduce plasmids into producer cells to generate viral particles.

Centrifugation / Purification: Clarify, concentrate, and purify the viral supernatant.

Quality Control & Delivery: Measure titer, verify purity, and provide the virus ready for use along with documentation.

APPLICATION OF VIRUS PACKAGING

Gene Delivery & Expression

Efficient, high-titer viral vectors for stable or transient gene delivery in cells.

Functional Genomics & Screening

CRISPR, sgRNA, or mutagenesis libraries for genome-wide or pathway-specific studies.

Disease Modeling & Experimental Research

Stable cell lines and in vivo models to study gene function and therapy.

HIGHLIGHTS OF SITE-DIRECTED MUTAGENESIS

self directed mutagenesis enables precise introduction of specific DNA mutations, including point mutations, insertions, or deletions. It is widely applied in protein functional site studies, enzyme activity optimization, DNA element functional analysis, and gene therapy research.

Tsingke provide professional and cost-effective site-directed mutagenesis services, accurately introducing desired mutations into any gene template at any number of sites, significantly enhancing experimental efficiency and reducing research costs.

SERVICE DETAILS OF SITE-DIRECTED MUTAGENESIS

Length Turnaround time (Calendar Day) Vector Deliverables

< 1.5 kb 7-10 Customer-specific (1) 1 tube of lyophilized plasmid DNA (about 1~4 μg/tube);

(2) QC files: Sequencing map (.abl file); Target sequence (.seq file); COA Report (elec tronic);

(3) Any mutations identified within a 30-base region will be considered as a single com prehensive mutation.

1.5kb~3kb 7-11

3kb~6kb 10-15

6kb~8kb 13-18

＞8kb Evaluation

siRNAs are 21-23 nt duplexes that guide RISC to cleave target mRNA. Tsingke provides conventional and modified siRNA from µg to kg scale.

HIGHLIGHTS OF sirna duplex

MS molecular weight detection with an error margin of less than 0.1% HPLC purity detection ensures purity levels greater than 90%;

A high modification rate is achieved, and stable groups such as Ps, 2-F, 2-OMe, and FAM are incorporated into the siRNA oligonucleotides to ensure suitability for downstream applications.

Leverage Tsingke's comprehensive industry chain advantages to provide fast and cost-effective services;

Multi-channel synthesizer with μg to kg level synthesis capabilities, synthesizing over 100,000 siRNA oligos annually.

SERService Details Length(nt) Quantity Purification QC Deliverable

siRNA Custom siRNA synthesis 20~25 5 nmol or 2 OD HPLC HPLC Tube or customized lyophilized RNA

COA report (electronic)

siRNA NC Various types available: negative/positive control siRNA, transfection control siRNA, etc 2.5 nmol/ 1OD

3 siRNA, 1 Guaranteed With a transfection efficiency of 90%, at least one out of the three siRNAs should achieve an mRNA interference efficiency of over 70% 5 nmol or 2 OD

4 siRNA, 1 Guaranteed With a transfection efficiency of 90%, at least one out of the four siRNAs should achieve an mRNA interference efficiency of over 70% 5 nmol or 2 OD

Large-scale siRNA Up to kg level ≥20 nmol or 8 O VICE DETAILS OF SIRNA DUPLEX

*Note: In addition to the recommended content, custom RNA oligo synthesis allows for the customization of length and purification methods.